New isozyme systems for maize (Zea mays L.): Aconitate hydratase, adenylate kinase, NADH dehydrogenase, and shikimate dehydrogenase
نویسندگان
چکیده
منابع مشابه
Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملMolecular Genetics of the Maize ( Zea mays 1 . ) Aspartate Kinase - H omoserine Dehydrogenase Gene Family ’ Gary
Aspartate kinase (AK) and homoserine dehydrogenase (HSDH) are enzymes in the aspartate-derived amino acid biosynthetic pathway. Recent biochemical evidence indicates that an AK-HSDH bifunctional enzyme exists in maize (Zea mays 1.). In this report, we characterize three genes that encode subunits of AK-HSDH. Two cDNAs, pAKHSDHl and pAKHSDH2, containing the fullcoding sequence, and one partial c...
متن کاملEffect of Exogenous Abscisic Acid on Proline Dehydrogenase Activity in Maize (Zea mays L.).
Plant responses to drought stress include proline and abscisic acid (ABA) accumulation. Proline dehydrogenase (PDH) (EC 1.4.3) is the first enzyme in the proline oxidation pathway, and its activity has been shown to decline in response to water stress (PJ Rayapati, CR Stewart [1991] Plant Physiol 95: 787-791). In this investigation, we determined whether ABA treatment affects PDH activity in a ...
متن کاملSolubilization of a Proline Dehydrogenase from Maize (Zea mays L.) Mitochondria.
L-Proline is oxidized to pyrroline-5-carboxylic acid in intact plant mitochondria by a proline dehydrogenase (EC 1.4.3) that is bound to the matrix side of the inner mitochondrial membrane (TE Elthon, CR Stewart [1981] Plant Physiol 67: 780-784). This investigation reports the first solubilization of the L-proline dehydrogenase (PDH) from plant mitochondria. The supernatant from NP-40-treated e...
متن کاملDuplicated cytosolic malate dehydrogenase genes in Zea mays.
Six inbred lines of Zea mays expressing different soluble (cytosolic) malate dehydrogenase (sMDH) zymogram phenotypes were analyzed genetically. sMDH was found to be coded for by unlinked duplicated loci in four of these inbred lines. The remaining two lines were found not to possess these duplicated loci. Furthermore, the duplicated loci, sMdh1 and sMdh2, have been found to be located on diffe...
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ژورنال
عنوان ژورنال: Biochemical Genetics
سال: 1988
ISSN: 0006-2928,1573-4927
DOI: 10.1007/bf02401795